LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(1619..1715) /label=attL2 /note="attL2 (c)" misc_signal complement(1730..1749) /note="T7 Promoter/priming site (c)" primer_bind 1757..1773 /note="M13 Reverse priming site" gene 1886..2695 /note="Kanamycin resistance gene" rep_origin 2816..3489 /note="pUC origin" vector join(1627..3492,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 669..691 /note="TEV site" source 692..1612 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:150894 IMAGE:40125836" /tissue_type="Colon, PCR rescued clones" /clone_lib="NIH_MGC_294" /note="Vector: pCR4-TOPO; Clone identification sequence tag: TCCCCTTA" gene 692..1612 /gene="ST8SIA1" /gene_synonym=GD3S /gene_synonym=ST8Sia I /gene_synonym=ST8SiaI /db_xref="GeneID:6489" /db_xref="HGNC:10869" /db_xref="MIM:601123" misc_feature 692..1612 /gene="ST8SIA1" /gene_synonym=GD3S /gene_synonym=ST8Sia I /gene_synonym=ST8SiaI /note="; This cDNA sequence aligns to the human reference genome (build 36) without differences" CDS 692..1612 /dnas_title="ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 1" /gene="ST8SIA1" /gene_synonym=GD3S /gene_synonym=ST8Sia I /gene_synonym=ST8SiaI /codon_start=1 /product="ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 1" /protein_id="AAI17286.1" /db_xref="GI:109659022" /db_xref="GeneID:6489" /db_xref="HGNC:10869" /db_xref="MIM:601123" /translation="MSPCGRARRQTSRGAMAVLAWKFPRTRLPMGASALCVVVLCWLY IFPVYRLPNEKEIVQGVLQQGTAWRRNQTAARAFRKQMEDCCDPAHLFAMTKMNSPMG KSMWYDGEFLYSFTIDNSTYSLFPQATPFQLPLKKCAVVGNGGILKKSGCGRQIDEAN FVMRCNLPPLSSEYTKDVGSKSQLVTANPSIIRQRFQNLLWSRKTFVDNMKIYNHSYI YMPAFSMKTGTEPSLRVYYTLSDVGANQTVLFANPNFLRSIGKFWKSRGIHAKRLSTG LFLVSAALGLCEEVAIYGFWPFSVNMHEQPISHHYYDNVLPFSGFHAMPEEFLQLWYL HKIGALRMQLDPCEDTSLQPTS" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg ccggctgccc aacgagaaag agatcgtgca 721 gggggtgctg caacagggca cggcgtggag gaggaaccag accgcggcca gagcgttcag 781 gaaacaaatg gaagactgct gcgaccctgc ccatctcttt gctatgacta aaatgaattc 841 ccctatgggg aagagcatgt ggtatgacgg ggagttttta tactcattca ccattgacaa 901 ttcaacttac tctctcttcc cacaggcaac cccattccag ctgccattga agaaatgcgc 961 ggtggtggga aatggtggga ttctgaagaa gagtggctgt ggccgtcaaa tagatgaagc 1021 aaattttgtc atgcgatgca atctccctcc tttgtcaagt gaatacacta aggatgttgg 1081 atccaaaagt cagttagtga cagctaatcc cagcataatt cggcaaaggt ttcagaacct 1141 tctgtggtcc agaaagacat ttgtggacaa catgaaaatt tataaccaca gttacatcta 1201 catgcctgcc ttttctatga agacaggaac agagccatct ttgagggttt attatacact 1261 gtcagatgtt ggtgccaatc aaacagtgct gtttgccaac cccaactttc tgcgtagcat 1321 tggaaagttc tggaaaagta gaggaatcca tgccaagcgc ctgtccacag gactttttct 1381 ggtgagcgca gctctgggtc tctgtgaaga ggtggccatc tatggcttct ggcccttctc 1441 tgtgaatatg catgagcagc ccatcagcca ccactactat gacaacgtct tacccttttc 1501 tggcttccat gccatgcccg aggaatttct ccaactctgg tatcttcata aaatcggtgc 1561 actgagaatg cagctggacc catgtgaaga tacctcactc cagcccactt ccTAGGACCC 1621 AGCTTTCTTG TACAAAGTTG GCATTATAAG AAAGCATTGC TTATCAATTT GTTGCAACGA 1681 ACAGGTCACT ATCAGTCAAA ATAAAATCAT TATTTGCCAT CCAGCTGATA TCCCCTATAG 1741 TGAGTCGTAT TACATGGTCA TAGCTGTTTC CTGGCAGCTC TGGCCCGTGT CTCAAAATCT 1801 CTGATGTTAC ATTGCACAAG ATAAAATAAT ATCATCATGA ACAATAAAAC TGTCTGCTTA 1861 CATAAACAGT AATACAAGGG GTGTTATGAG CCATATTCAA CGGGAAACGT CGAGGCCGCG 1921 ATTAAATTCC AACATGGATG CTGATTTATA TGGGTATAAA TGGGCTCGCG ATAATGTCGG 1981 GCAATCAGGT GCGACAATCT ATCGCTTGTA TGGGAAGCCC GATGCGCCAG AGTTGTTTCT 2041 GAAACATGGC AAAGGTAGCG TTGCCAATGA TGTTACAGAT GAGATGGTCA GACTAAACTG 2101 GCTGACGGAA TTTATGCCTC TTCCGACCAT CAAGCATTTT ATCCGTACTC CTGATGATGC 2161 ATGGTTACTC ACCACTGCGA TCCCCGGAAA AACAGCATTC CAGGTATTAG AAGAATATCC 2221 TGATTCAGGT GAAAATATTG TTGATGCGCT GGCAGTGTTC CTGCGCCGGT TGCATTCGAT 2281 TCCTGTTTGT AATTGTCCTT TTAACAGCGA TCGCGTATTT CGTCTCGCTC AGGCGCAATC 2341 ACGAATGAAT AACGGTTTGG TTGATGCGAG TGATTTTGAT GACGAGCGTA ATGGCTGGCC 2401 TGTTGAACAA GTCTGGAAAG AAATGCATAA ACTTTTGCCA TTCTCACCGG ATTCAGTCGT 2461 CACTCATGGT GATTTCTCAC TTGATAACCT TATTTTTGAC GAGGGGAAAT TAATAGGTTG 2521 TATTGATGTT GGACGAGTCG GAATCGCAGA CCGATACCAG GATCTTGCCA TCCTATGGAA 2581 CTGCCTCGGT GAGTTTTCTC CTTCATTACA GAAACGGCTT TTTCAAAAAT ATGGTATTGA 2641 TAATCCTGAT ATGAATAAAT TGCAGTTTCA TTTGATGCTC GATGAGTTTT TCTAATCAGA 2701 ATTGGTTAAT TGGTTGTAAC ACTGGCAGAG CATTACGCTG ACTTGACGGG ACGGCGCAAG 2761 CTCATGACCA AAATCCCTTA ACGTGAGTTA CGCGTCGTTC CACTGAGCGT CAGACCCCGT 2821 AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA 2881 AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT 2941 TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTTC TTCTAGTGTA 3001 GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT 3061 AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC 3121 AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA 3181 GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA 3241 AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG 3301 AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT 3361 CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG 3421 CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT 3481 TGCTCACATG TT //